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Episode
The Skin Longevity Breakthrough | Carolina Reis Oliveira
~74 min
Episode Brief·YouTube

The Skin Longevity Breakthrough | Carolina Reis Oliveira

Kara Fitzgerald
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TL;DR

The four things you'd lose by not watching

4 items

TL;DR

The four things you'd lose by not watching

4 items
1

OneSkin's OS-01 peptide reduces senescent cells by 20-50%, matching the potency of rapamycin in lab models without the immunosuppressive side effects, and a 12-month pilot study showed a 3.3-year reduction in skin biological age.

2

A 3-month clinical study demonstrated that topical application of OneSkin products significantly lowered systemic inflammation (specifically IL-8) in adults aged 60-85, proving skin care can influence whole-body health.

3

The company screened over 900 peptides over 5 years to find OS-01, and they built their own skin-specific epigenetic clock to validate biological age reversal, moving beyond subjective 'looks better' claims.

4

Carolina critiques the skincare industry for relying on ingredient supplier data rather than testing the final formulation, warning that combining actives often reduces efficacy rather than creating synergy.

Protocols

Concrete recipes — what, when, how much, and why

5 items

Combining OneSkin with Retinol

WhatUse OneSkin moisturizer in conjunction with retinol to mitigate irritation and improve tolerance.
WhenDaily, as part of your morning or evening routine.
For whomAnyone using retinol, particularly those with sensitive skin who struggle with retinol irritation.
WhyRetinol causes cell renewal that can lead to inflammation and irritation. OS-01 reduces inflammation and supports barrier repair, counteracting these side effects.
CaveatsOrder of application depends on tolerance.

Carolina explains that retinol is potent but many people cannot tolerate it due to the irritation it causes. OneSkin can be used as a buffer. For those with high sensitivity, applying OneSkin first creates a protective base. For those with established tolerance, applying retinol first allows it to work, followed by OneSkin to soothe and repair. She hints at a future product specifically designed to address retinol's drawbacks while preserving its benefits.

Mechanism

Retinol accelerates skin cell turnover, which can compromise the skin barrier and trigger inflammatory pathways. OS-01 reduces the senescent cell burden and associated SASP inflammation, helping to calm the skin and restore barrier function, allowing the user to tolerate the retinoid longer and with less irritation.

if you use our product, you know, in conjunction with retinol, you can tolerate better. You can um even reduce the the potential irritation.

Also said
“if you're someone that has um more sensitivity to retinol, you can apply one skin first and then apply retinol on top.”— Provides specific actionable protocol for sensitive users.
“If you're someone that already tolerates retinol well, you can apply retinol first and then one skin on top.”— Provides specific actionable protocol for tolerant users.

Using OneSkin with Microneedling and Lasers

WhatPrep skin with OneSkin before procedures, and apply after wounds are closed to speed recovery.
WhenPre-treatment for months, post-treatment after wound closure.
For whomIndividuals undergoing microneedling, laser treatments, or similar cosmetic procedures.
WhyStrengthens skin resilience pre-procedure, speeds recovery and reduces inflammation post-procedure.
CaveatsDo not apply on open wounds. Apply once the wound is closed.

Carolina details the customer reports and her own experience. The peptide penetrates intact skin, so it's effective without microneedling, but it aids recovery when used after the skin is closed. Using it for a few months prior strengthens the skin, making it more resilient to the 'insult' of the procedure. Post-procedure, it helps reduce downtime, inflammation, and redness.

Personal experience

Carolina attests to this herself, noting faster recovery and less inflammation when using OneSkin before and after procedures.

it helps with, like, the micro after the microneedling or the laser, like, the downtime to to reduce the inflammation, to reduce the redness.

Also said
“a prep with one skin for a few months, we see a lot of customers reporting that, and I I can attest to that myself.”— Confirms the pre-treatment benefit through both customer reports and personal experience.

Using OneSkin for GLP-1 Weight Loss

WhatUse OneSkin body lotion and face products during and after GLP-1 agonist use to maintain skin quality.
WhenDuring the weight loss phase and maintenance.
For whomPatients using GLP-1 agonists (e.g., Ozempic, Wegovy) who are concerned about facial aging or skin sagging.
WhyTo maintain skin quality, barrier function, and collagen production while the underlying adipose layer is shrinking.
CaveatsNot a substitute for proper nutrition and gradual weight loss. A clinical study is currently underway to formally validate this application.

Carolina explains that the idea came from customer reports. They are now launching a clinical study with patients in the stable phase of GLP-1 use. The body lotion is particularly relevant as it strengthens the barrier and supports collagen production across the whole body, which is undergoing significant change.

Mechanism

Rapid weight loss reduces the protective adipose stem cell layer under the skin, removing structural support and anti-inflammatory factors. OneSkin provides the building blocks (collagen support, barrier repair) that the skin is losing during this process.

we already know that the body lotion helps like with the uh strengthening the barrier of the skin and helps with collagen production. So, we know that it's already providing that, again, those building blocks that you may be losing during this process of weight loss.

Also said
“if you lose too fast, you're going to see a very significant impact in the quality of the skin.”— Highlights the importance of the rate of weight loss as a factor in skin quality.

Using OneSkin for Wound Healing and Scarring

WhatApply OneSkin body lotion or face product around a closed wound or healing scar to modulate inflammation and improve matrix remodeling.
WhenAfter the wound has closed. Do not apply on open wounds.
For whomIndividuals with healing wounds, post-surgical scars (e.g., C-sections), or diabetic patients with slow-healing wounds.
WhyPersistent senescent cells at the wound site can cause excessive inflammation and fibrosis (scarring). OS-01 reduces the senescent cell burden, helping to normalize the healing process.
CaveatsApply around the wound, not directly on the open wound. For diabetic wounds, apply to the surrounding skin to support the healing environment.

Carolina explains the dual role of senescent cells in wound healing. They are initially necessary to recruit immune cells to close the wound. However, if they persist, they secrete SASP factors that impair the remodeling of the extracellular matrix, leading to fibrosis and poor scarring. By applying OneSkin after the wound is closed, the OS-01 peptide can help modulate the senescent cell population, leading to a smoother, more natural matrix deposition. She shares anecdotal reports from customers using it on C-section scars and diabetic patients using it to help close persistent wounds.

Mechanism

Senescent cells are initially beneficial for wound closure (immune recruitment). If they persist, they cause chronic inflammation (SASP) which disrupts the balance of collagen deposition and degradation, leading to scar formation. OS-01 reduces the number of these persistent senescent cells and lowers SASP inflammation, allowing the normal remodeling process to proceed more effectively.

right after you close the wound, applying our products... it can help basically having a more smooth like, you know, matrix deposition by, you know, uh modulating the senescent cells factor secretion.

Also said
“senescent cells are actually important to help close the wound. So, the senescent cells will basically recruit like the immune cells that will help like close that wound.”— Provides the crucial context that senescence is not universally bad; timing is everything.
“we saw people that had like difficulties with closing wound and in this case are like more diabetic patients... they applied uh the OneSkin around the wound and that helped like close the wound too.”— Provides a specific, high-impact use case (diabetic wounds) that validates the mechanism.

Using OneSkin Hair Serum

WhatApply the water-based serum to the scalp, optionally using a derma roller for enhanced penetration.
WhenOnce or twice daily.
DoseConsistent use for 3-6 months to see results.
For whomIndividuals experiencing age-related hair thinning (common after 50-60), postpartum hair loss, or menopause-related hair loss.
WhyTargets age-related hair thinning by reducing senescent cell burden in the hair follicle, increasing hair density and thickness by 30-40%.
CaveatsRequires consistency. Results take time. The derma roller is optional but can improve penetration.

Carolina explains the hypothesis that the scalp is a continuation of the skin and suffers from the same senescent cell accumulation. The serum is designed for age-related hair thinning, not sudden patchy hair loss. It is safe for use during fertility and postpartum, unlike minoxidil which she personally avoids during her 'fertility phase'.

Mechanism

Senescent cells accumulate in the hair follicle and produce inflammation, which is a contributing factor to age-related hair thinning and loss. The OS-01 peptide reduces these senescent cells and the associated inflammation, creating a healthier environment for hair growth.

Personal experience

Carolina uses it herself and reports significant new hair growth. She cannot use minoxidil due to trying to conceive, making this a safe alternative for her.

we saw in our studies that they helps like increasing hair density and hair thickening thickness about, you know, 30 to 40%.

Also said
“I can't take, you know, right now my minoxidil, you know, I'm still in my fertility um phase and so this is a safe formula”— Highlights a key safety advantage over a standard treatment (minoxidil) for a specific population.

What's new

Personal practice updates, fresh positions, predictions

5 items

OS-01 peptide discovery and senolytic potency

OneSkin's OS-01 peptide reduces senescent cells by 20-50%, matching the potency of rapamycin in lab models without the immunosuppressive side effects.

Why this matters: Positions a topical cosmetic peptide as having comparable efficacy to a potent pharmaceutical senolytic (rapamycin), challenging the ceiling of what topical skincare can achieve.

Background

Most anti-aging skincare targets surface-level effects (retinoids, acids). OneSkin aimed to target the root cause: cellular senescence.

Carolina explains that they screened over 900 peptides over 5 years. The goal was to find a molecule that could reduce the burden of senescent cells, which are damaged cells that secrete inflammatory factors (SASP) and drive aging. They tested OS-01 against known senolytics like fisetin, NMN, metformin, and rapamycin. OS-01 consistently matched or exceeded the senescent cell reduction of rapamycin (20-50% reduction) without the potential for immune compromise associated with rapamycin. This was a pivotal finding that validated their entire approach.

Our peptide very often compares to the rapamycin effect in terms of the reducing senescent but without the potential side effects immuno compromise that can happen with rapamycin.

Also said
“We spent over 5 years just screening hundreds of peptides, and eventually we landed into OS-1.”— Establishes the rigorous, long-term R&D effort behind the discovery.
“it can basically reduce the level of senescent cells, you know, from 20 to 50% in our studies.”— Provides the specific quantitative efficacy range of the peptide.

Topical skincare can lower systemic inflammation

A 3-month clinical study showed that using OneSkin face and body lotion significantly lowered the systemic inflammatory marker IL-8 in adults aged 60-85.

Why this matters: Provides direct evidence that a topical intervention can influence whole-body health, bridging the gap between dermatology and systemic longevity medicine.

Background

The skin is the largest organ. As it ages, its barrier weakens, becoming a source of low-grade inflammation that can impact internal health (e.g., psoriasis patients have higher cardiovascular risk).

Carolina details the study: participants aged 60-85 used OneSkin face and body lotion twice daily for 3 months. A control group used an emollient-only product. The OneSkin group saw a ~40% increase in skin hydration and barrier function, over 70% saw improved skin quality, and critically, their blood showed a significant decrease in IL-8, a key pro-inflammatory cytokine. The control group did not see these improvements; some markers increased. This suggests that repairing the skin barrier with the OS-01 peptide can dampen systemic inflammation.

We saw a decrease in several markers related uh associated to inflammation and a significant drop in uh the I interleukin 8.

Also said
“people with psoriasis have around like 50% more chances to have um cardiovascular disease.”— Provides context on the established link between skin inflammation and systemic disease.
“the control did not decrease. They actually some of the markers were increased in the control.”— Highlights that the control product (emollients) was not just neutral but potentially detrimental, emphasizing the active benefit of OS-01.

GLP-1 accelerated facial aging is an active area of investigation

OneSkin is launching a clinical study to see if their OS-01 peptide can mitigate the skin sagging and quality loss associated with rapid weight loss from GLP-1 agonists.

Why this matters: Addresses a major emerging side effect of a hugely popular drug class, potentially offering a topical solution to a systemic problem.

Background

Rapid weight loss reduces the adipose stem cell layer under the skin, removing structural support and anti-inflammatory factors.

Carolina explains the mechanism of facial aging on GLP-1s: rapid loss of the protective adipose stem cell layer under the skin. This layer provides structure and anti-inflammatory factors. OneSkin's role is to provide the building blocks the skin is losing during this process. A clinical study is underway focusing on patients in the stable phase of GLP-1 use to measure the impact of OneSkin on skin quality.

if you lose too fast, you're going to see a very significant impact in the quality of the skin. And in our product, our hope is that we can minimize some of those effects.

Also said
“under the skin there is a layer of like a deep osseous stem cells... you lose like the the structure, right?”— Explains the anatomical mechanism behind the skin changes during rapid weight loss.

The skincare industry needs to test final formulations

Most brands rely on ingredient supplier data, but combining ingredients can negate or reduce efficacy. OneSkin tests every final formula in vitro.

Why this matters: Directly challenges standard industry practice and explains why many 'active' products underperform despite having good ingredients.

Background

The industry standard is to formulate based on ingredient supplier claims without testing the final mixture on skin models.

Carolina explains that increasing the dose of OS-01 or combining it with other actives doesn't always improve results. The final formula must be tested on skin models to ensure it doesn't cause toxicity or reduce efficacy. This is a core part of their R&D philosophy. She notes that even combining two different peptides or adding too many actives can result in a final product that performs worse than the sum of its parts.

Most of the times what we think that's synergistic is not. So, 1 + 1 doesn't equal two or three or four or sometimes it equal like one and a half or one.

Also said
“we have test like putting like different peptides combining two OS1 or even increasing the dose of OS1 and it's not necessarily better.”— Provides a concrete example of how their testing revealed non-synergistic results.

Senescent cells are essential for wound healing but detrimental if persistent

Acute inflammation driven by senescent cells is necessary to close a wound, but if they persist, they cause scarring and fibrosis.

Why this matters: Nuances the 'senescent cells are bad' narrative, explaining the therapeutic window for interventions like OS-01.

Background

Senescent cells are typically viewed as purely detrimental drivers of aging.

Carolina explains the dual role: senescent cells are initially necessary to recruit immune cells to close the wound. However, if they persist, they secrete SASP factors that impair the remodeling of the extracellular matrix, leading to fibrosis and poor scarring. By applying OneSkin after the wound is closed, the OS-01 peptide can help modulate the senescent cell population, leading to a smoother, more natural matrix deposition. She shares anecdotal reports from customers using it on C-section scars and diabetic patients using it to help close persistent wounds.

senescent cells are actually important to help close the wound... but you don't want to have persistent senescent cells there because if they they continue to secrete inflammation, they can impair like the remodeling of the matrix.

Also said
“we saw people that had like difficulties with closing wound and in this case are like more diabetic patients... they applied uh the OneSkin around the wound and that helped like close the wound too.”— Provides a specific, high-impact use case (diabetic wounds) that validates the mechanism.

Recommendations

Products, supplements, and tools mentioned in the episode

1 item

Scrutinize Skincare Brands for Final Formulation Testing

Practice

Carolina recommends consumers and clinicians ask brands for in vitro and clinical studies of their final formulation, not just ingredient data.

Carolina explains that the industry standard is to formulate based on ingredient supplier claims without testing the final mixture on skin models. She warns that combining ingredients can reduce efficacy or cause toxicity. She advises asking brands directly: 'What kind of test have you done? Are there any third parties that have evaluated your product in terms of safety or efficacy?' This pushes the industry towards greater accountability.

vs alternatives

This practice separates brands that do rigorous final formulation testing from those that rely solely on ingredient supplier data and subjective consumer studies.

go to the brand, ask for the studies that they have done. They should have done, you know, ideally in vitro studies, clinical studies, published like by peer-reviewed uh journals.

Also said
“what we have found in our research that very few companies do and that what it's really important is testing the final formula”— Establishes that this rigorous testing is rare in the industry, making it a key differentiator.
Find Scrutinize
Disclosed sponsorships5speaker disclosed

OneSkin OS-01 Face Moisturizer

Product Sponsored · disclosed

The foundational OneSkin product containing the OS-01 peptide and three types of hyaluronic acid.

DisclosureCarolina is the CEO and co-founder of OneSkin.

This is the foundational OneSkin product. It contains the OS-01 peptide which was discovered after screening over 900 peptides. In lab (in vitro) studies, treatment with the peptide for 5 days reduced the biological age of skin samples by 2.5 years according to their proprietary epigenetic clock. A 12-month pilot study in humans showed a 3.3-year reduction in skin biological age. The peptide reduces senescent cells by 20-50%, stimulates collagen production, and restores the skin barrier. The formula also includes three types of hyaluronic acid for deep hydration.

vs alternatives

Unlike standard anti-aging products that focus on surface renewal (retinoids, acids) without targeting root causes of aging like cellular senescence.

it can basically reduce the level of senescent cells, you know, from 20 to 50% in our studies. And really, as I said, shut down inflammation, basically help your skin cells to replicate again, produce collagen, restore the barrier, and bring the skin to that younger state.

Also said
“We published that the clock back in 2020. So, it's been like almost six years and we also published the data that we found that our peptide could reduce the age of the skins that we treated in the lab in 2.5 years”— Provides the specific data point for biological age reversal in vitro.
Find OneSkin

OneSkin OS-01 Eye Cream

Product Sponsored · disclosed

The most potent formula in the OneSkin line, with the highest concentration of the OS-01 peptide. Tested specifically on eyelid skin.

DisclosureCarolina is the CEO and co-founder of OneSkin.

Carolina explains that they were able to increase the peptide concentration in this formula because the specific combination of ingredients for the eye area allowed it. It is designed for the delicate eye area but can be used on other target areas like mouth lines, forehead wrinkles, and the neck. Clinical studies showed significant smoothing of eyelid wrinkles and increased hydration measured by instruments. It increased collagen by 130% more than the face product in lab tests.

vs alternatives

Unlike standard eye creams that focus on hydration or temporary tightening, this targets the underlying senescent cell burden and collagen production at a higher potency.

Personal experience

Carolina uses it around her eyes, mouth lines, and forehead. Kara uses it on her cheeks and around her lips in addition to the eye area.

I apply around my eyes and then I apply around, you know, my mouth lines and if I have some lines on my forehead, any you know, target area.

Also said
“it actually reduced the age markers that we measure in a more significant way.”— Confirms the higher potency translates to better biological age reversal metrics.
Find OneSkin

OneSkin OS-01 Body Lotion

Product Sponsored · disclosed

Contains the OS-01 peptide and ceramides. Used in the landmark systemic inflammation study.

DisclosureCarolina is the CEO and co-founder of OneSkin.

This product was used in the landmark 3-month clinical study on adults aged 60-85. It contains the OS-01 peptide along with ceramides to support the skin barrier. The study showed a 40% increase in skin hydration and barrier function, over 70% of participants saw improved skin quality, and critically, there was a significant drop in the systemic inflammatory marker IL-8 in the blood. This provides evidence that repairing the skin barrier topically can lower whole-body inflammation.

vs alternatives

Unlike standard body lotions that only provide emollient hydration without active senolytic or barrier-repairing ingredients.

We saw uh around 40% increase in in hydration and barrier function. And over 70% of our of our uh participants saw an improvement in the overall skin quality.

Also said
“we saw a decrease in several markers related uh associated to inflammation and a significant drop in uh the I interleukin 8.”— Provides the specific systemic anti-inflammatory data from the clinical study.
Find OneSkin

OneSkin OS-01 Sunscreen (Tinted)

Product Sponsored · disclosed

A tinted mineral sunscreen containing the OS-01 peptide and five potent antioxidants (vitamin C, green tea, acai).

DisclosureCarolina is the CEO and co-founder of OneSkin.

This is a tinted mineral sunscreen that also functions as a treatment product. It contains the OS-01 peptide along with five potent antioxidants: a stable form of vitamin C, green tea, and acai extract. Carolina recommends it as a replacement for a separate vitamin C serum, simplifying the morning routine. It provides broad-spectrum sun protection while continuously delivering the anti-aging and barrier-supporting benefits of the peptide. Kara notes it gives a healthy glow and can replace foundation or blush.

vs alternatives

Unlike standard sunscreens that only block UV without actively repairing skin and providing antioxidants.

Personal experience

Kara uses it as a replacement for blush and foundation. Carolina uses it as her sole morning antioxidant and peptide source.

it also has like five very potent antioxidants. So, we have like a very stable form of vitamin C, we have green tea, we have acai extract. And so, this can replace your vitamin C serum.

Also said
“The tinted face um sunscreen has completely taken over any you know, any makeup I would be using. Like, you know, that's a I'm not using blush. I'm not foundation, right?”— Demonstrates a real-world aesthetic benefit that simplifies a user's routine.
Find OneSkin

OneSkin OS-01 Hair Serum

Product Sponsored · disclosed

A water-based serum for age-related hair thinning, designed to be used with or without a derma roller.

DisclosureCarolina is the CEO and co-founder of OneSkin.

The serum targets age-related hair thinning by reducing senescent cells in the hair follicle. Studies showed a 30-40% increase in hair density and thickness. It is safe for use during fertility and postpartum, unlike minoxidil. The formula is water-based and can be used with a derma roller for enhanced penetration into the scalp.

vs alternatives

Unlike standard hair loss treatments like minoxidil, which may not be suitable for everyone (e.g., those trying to conceive) and don't target the senescent cell mechanism.

Personal experience

Carolina uses it and reports significant regrowth. She specifically values it as a safe alternative to minoxidil during her fertility phase.

we saw in our studies that they helps like increasing hair density and hair thickening thickness about, you know, 30 to 40%.

Also said
“I can't take, you know, right now my minoxidil, you know, I'm still in my fertility um phase and so this is a safe formula”— Highlights a key safety advantage over a standard treatment (minoxidil) for a specific population.
Find OneSkin

Notable quotes

Lines worth pulling out — contrarian, specific, or perfectly phrased

5 items
Our peptide very often compares to the rapamycin effect in terms of the reducing senescent but without the potential side effects immuno compromise that can happen with rapamycin.
Positions a topical cosmetic peptide as having comparable efficacy to a potent pharmaceutical senolytic, challenging the ceiling of what topical skincare can achieve.
We saw a decrease in several markers related uh associated to inflammation and a significant drop in uh the I interleukin 8.
Hard data proving a topical product can lower systemic inflammation, a game-changing concept for the skincare and longevity fields.
Most of the times what we think that's synergistic is not. So, 1 + 1 doesn't equal two or three or four or sometimes it equal like one and a half or one.
Contrarian view on formulation, challenging a core assumption in the supplement/skincare industry that more active ingredients are always better.
the skin is a more accessible tissue and um if we can correlate some of those skin changes and and biomarkers with you know what's going on internally, I think this will be a very interesting way to tap into the skin more as a as a way to understand what's going on internally.
Visionary statement on the future of skin as a non-invasive biomarker for systemic health and longevity.
We spent over 5 years just screening hundreds of peptides, and eventually we landed into OS-1.
Establishes the immense R&D effort and scientific rigor behind the single active ingredient, setting the company apart from typical skincare brands.

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Topics covered

cellular-senescencesasp-inflammationos-01-peptidesenolytic-screeningepigenetic-clockskin-biological-ageskin-barrier-repairsystemic-inflammationil-8glp-1-agonist-skin-healthwound-healingscar-modulationhair-thinningage-related-hair-lossretinol-tolerancemicroneedling-recoveryproduct-formulation-testingskincare-industry-critiquelongevity-medicinetopical-supplement
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